TBA Total Bile Acid Assay Kit Principle of Enzyme Cycle Method

Total bile acid (TBA) is an important indicator for the evaluation of liver disease and digestive system function, and its detection is of great significance in clinical medicine. The TBA detection kit (enzyme cycle method) has become one of the main means of detecting TBA with its high sensitivity, high specificity and simple operation.

The basic principle of the enzyme cycle method of the TBA detection kit is to convert trace amounts of bile acid into a large amount of detectable products through enzyme cycle amplification technology, thereby realizing the quantitative detection of TBA. This method is mainly divided into the following three steps.

1. Conversion reaction: In this step, bile acid, as a substrate, is converted into a circulating substrate through the action of specific enzymes. This circulating substrate plays a key role in the subsequent circulating reaction.
2. Cycle reaction: The generated circulating substrate participates in a coupling reaction consisting of two enzyme reactions, one of which converts the circulating substrate into an intermediate product, and the other enzyme converts the intermediate product into bile acid and NAD+ (oxidized coenzyme I). The two enzyme reactions are cyclic, so that bile acid is continuously amplified, thereby improving the sensitivity of detection.
3. Indicator reaction: By measuring the absorbance change of NADH (reduced coenzyme I) at a specific wavelength (such as 405nm), the concentration of TBA can be indirectly calculated. Since the amount of NADH generated is proportional to the concentration of TBA, the quantitative detection of TBA can be achieved through the standard curve.

The enzyme cycle method of the TBA detection kit has a wide range of application prospects in the field of TBA detection due to its high sensitivity, high specificity and simple operation. By deeply understanding its principles and characteristics, we can better use this method for quantitative detection of TBA, providing strong support for the diagnosis and treatment of liver diseases and digestive system diseases.