ALP Alkaline Phosphatase Assay Kit Overview of ALP continuous monitoring method

In biochemistry and clinical testing, the determination of alkaline phosphatase (ALP) is of great significance for the diagnosis and monitoring of various diseases. The continuous monitoring method of ALP Assay Kit is an efficient and accurate method for in vitro quantitative detection of ALP activity in serum or cell culture supernatant.

The continuous monitoring method is mainly based on the enzymatic reaction kinetics of ALP. Under alkaline conditions, ALP can catalyze the hydrolysis of phosphate substrates to generate phosphoric acid and corresponding alcohols. During the reaction, the substrate concentration gradually decreases, while the product concentration gradually increases. By continuously monitoring the changes in product or substrate concentration during the reaction, the activity of ALP can be calculated. In actual operation, commonly used substrates include p-nitrophenol phosphate (p-NPP) and disodium phenyl phosphate. Taking p-NPP as an example, ALP catalyzes its hydrolysis to generate p-nitrophenol (p-NP). p-NP is yellow under alkaline conditions, and its absorbance is proportional to the concentration. Therefore, by continuously monitoring the absorbance changes of p-NP in the reaction system, the activity of ALP can be indirectly calculated.

The continuous monitoring method of ALP Assay Kit is widely used in clinical diagnosis and scientific research. In clinical diagnosis, this method can be used to assist in the diagnosis of liver and gallbladder diseases, bone diseases, etc. For example, in liver and gallbladder diseases, ALP activity often increases, which can be used as a diagnostic and monitoring indicator of the disease. In scientific research, this method can be used to study the mechanism of action of ALP in cell signal transduction, metabolic regulation, etc.