Calcium Assay Kits and Arsenazo Method

A calcium assay kit can measure intracellular calcium changes in living cells in a high-throughput environment. BD's Calcium Assay Kit involves only one dye addition step, which makes it easy to implement in a high-throughput environment. However, it is important to remember that cell numbers should be optimized for each assay, ideally in a confluent monolayer. You must keep diluted standards at 4degC for future use.

The Fluo-4 Direct Calcium Assay Kit is designed to measure intracellular calcium mobilisation using a fluorogenic dye. It is optimized for superior cell permeability and retention, and features a self-quenching dye that minimizes background fluorescence. Therefore, it is ideal for use in a cell culture system. The Fluo-4 Direct Calcium Assay Kit can be used in cell cultures and can also be applied to culture media.

The Kangte Calcium Assay Kit measures calcium ions in human serum in vitro. It is used for the auxiliary diagnosis of hyperparathyroidism, rickets, vitamin D deficiency, and tetany. It also detects the presence of hydroxyproline, a substance found in human serum. In this way, the Assay Kit enables physicians to accurately diagnose conditions involving increased calcium.

The Calcium Assay Kit is a simple and convenient way to determine calcium in biological samples. The test uses a red fluorescence probe that is read with a fluorescence microplate reader. The assay is fast and easy, requiring less than 30 minutes of work. The kit also has an excellent dynamic range. It is ideal for clinical and research use, and provides information on the effects of drugs and hormone imbalances. It's also useful for monitoring calcium level in human tissues.

When working with a calcium assay kit, make sure to dilute the working reagent in water or alcohol. If there is a high concentration of proteins or lipids in the solution, this will affect the observed value. To remove these contaminants, perform a centrifugation process. Alternatively, dilute the samples in equal volumes of Reagent A and Reagent B. After mixing, ensure the reagents are at room temperature before use.

The Calcium Indicator can be stored at -20degC and the 1X PBX Signal Enhancer can be stored at room temperature. Once reconstituted, the Calcium Indicator will remain stable for over one month. To avoid repeat freeze-thaw cycles, tightly seal the tubes and place them in a desiccant-containing zip lock bag. Once the Calcium Assay Kit is ready to use, prepare the 1X Dye-loading Solution.

This assay kit enables fluorescence-based measurements of intracellular calcium mobilization. Flow-cytometric calcium assays can also be used in cell sorting. This kit uses a calcium-sensitive dye that can cross the cell membrane. Calcium binding enhances the dye's fluorescence. The result is a fluorescence response that is proportional to the amount of intracellular calcium released. When calcium-binding cells express an active GPCR, intracellular calcium is released.