How to use myocardial specific proteins clinical diagnostic reagent assay kit

The Myocardial Specific Proteins Clinical Diagnostic Reagent Assay Kit is a high-end diagnostic tool designed specifically for detecting and analyzing the content of specific proteins associated with myocardial damage in the blood. These specific proteins include cardiac troponins (such as cTnI and cTnT), myoglobin (Myo), and creatine kinase isoenzyme (CK-MB). These proteins are released into the blood when the myocardium is damaged, so their detection has important clinical value in the diagnosis of cardiovascular diseases, especially in the early identification of diseases such as acute myocardial infarction (AMI).

The use of this kit follows strict operating procedures and standardized operating specifications to ensure the accuracy and reliability of the test results. The following is a detailed description of the use of the kit:

Before using the Myocardial Specific Proteins Clinical Diagnostic Reagent Assay Kit, relevant equipment and reagents need to be prepared. This includes a clean, dry and non-contaminated disposable sample collection container for collecting blood samples from patients; a timer is used for time control during the test process. In addition, the kit, serum samples to be tested, and quality control references need to be restored to room temperature (generally 15-30°C) to ensure the accuracy of the test.

Sample collection and pretreatment are important links in the entire testing process. Usually, the patient's venous blood sample is collected using an anticoagulant tube, and then centrifuged according to the requirements of the kit instructions to separate serum or plasma as the test sample. When collecting and pre-treating samples, aseptic operation specifications should be strictly followed to avoid sample contamination and cross-infection.

After the sample is processed, take out the test card and mark the patient or sample number according to the requirements of the kit instructions. Place the test card on a stable table and prepare for sample addition. When adding samples, use a sample pipette to absorb an appropriate amount of serum sample and drip it vertically on the sample addition area of ​​the test card. After the sample is added, start the timer immediately and interpret the results according to the time range required by the instructions (generally 10-20 minutes).

During the result interpretation process, the color change on the test card needs to be carefully observed. Myocardial specific protein clinical diagnostic kits usually use immunoturbidimetry or enzyme-linked immunosorbent assay (ELISA) and other technologies for detection. These methods are based on the principle of antigen-antibody reaction and quantitatively detect their content by measuring the degree of binding between specific proteins in the sample and antibodies in the reagent. The color change on the test card reflects the content of specific protein in the sample, and the darker the color, the higher the content.