ADA Principle of Adenosine Deaminase Assay Kit (Glutamate Dehydrogenase Method)

In the field of modern medical diagnosis, the measurement of biochemical indicators plays a vital role in the diagnosis, treatment and prognosis assessment of diseases. Among them, adenosine deaminase (ADA), as an important enzyme marker, plays an indispensable role in the diagnosis of various diseases. ADA is usually measured using a specific kit. Here we introduce in detail the principle of the ADA Adenosine Deaminase Assay Kit based on the glutamate dehydrogenase method.

1. The physiological significance of ADA
ADA is an enzyme widely present in various tissues of the human body, especially in lymphocytes. It is mainly involved in the metabolic process of adenosine, converting adenosine into inosine and ammonia. Under normal physiological conditions, ADA activity remains at a relatively stable level. However, when certain diseases occur in the human body, the activity of ADA will change. For example, in diseases such as tuberculous pleurisy, hepatitis, and cirrhosis, the activity of ADA will increase significantly.

2. Principle of ADA determination kit
The principle of the ADA assay kit (glutamic acid dehydrogenase method) is based on ADA catalyzing the deamination of adenosine to generate inosine and ammonia, and then using glutamate dehydrogenase to convert ammonia into glutamate, simultaneously producing NADH. NADH has a characteristic absorbance at a wavelength of 340nm. By measuring the change in absorbance, the activity of ADA can be indirectly calculated.
Specifically, the kit contains reaction substrate adenosine, coenzyme NADH, glutamate dehydrogenase and other necessary reagents. During the reaction, ADA catalyzes the deamination of adenosine to generate ammonia, which reacts with α-ketoglutarate under the action of glutamate dehydrogenase to generate glutamate, while consuming NADH. By measuring the absorbance change of NADH before and after the reaction, the activity of ADA can be calculated.

ADA determination kit (glutamic acid dehydrogenase method) is a sensitive, accurate and convenient ADA determination method. It is based on the principle that ADA catalyzes the deamination of adenosine to generate ammonia, and calculates the activity of ADA by measuring the absorbance change of NADH. This kit has broad application prospects in clinical medicine and is of great significance for improving the diagnostic accuracy of diseases, guiding treatment and prognosis assessment.