ABOUT Cystatin C Assay Kit

Human Cystatin C is a soluble protein found in the urinary tract. Its levels are higher than those in the urine, but they are significantly lower than those in the blood. The Mouse Cystatin C ELISA Assay Kit is used to determine the concentration of cystatin in biological samples. It is particularly useful in assessing the level of cystatin in samples obtained from mice. The results can be read out in 90 minutes.

The Cystatin C Assay Kit contains a standard solution, which is composed of 15 ng of native human Cystatin C in a buffered protein base. This standard is stable and may be stored at -20 oC for up to one month. However, it must not be subjected to repeated freeze/thaw cycles. Similarly, the Wash Buffer consists of 30 mL of 10x wash buffer and 270 mL of diH 2 O.

The Cystatin C Assay Kit is a quantitative sandwich enzyme immunoassay (ELISA) based on an absorbance method. The sample is pipetted into wells precoated with an anti-human Cystatin C antibody. The sample is then exposed to a TMB solution. The TMB solution develops a color in proportion to the amount of human CYSTATIN C initially bound. Finally, the samples are incubated with an acidifying solution, which stops the color development. Optical densities are determined by plotting standard curves against the measured absorbance. The concentration of the antigen is reported in ng/mL.
 

The Cystatin C Assay Kit includes a standard and a reagent. The standard is 15 ng of native human Cystatin C in a buffered protein base. The sample should be diluted with 1 mL of 1x Assay Buffer and stored at -20 o C for a month. The Cystatin c Assay Kit contains a sample reagent, which is 30 mL of 10x wash buffer. The reconstituted sample must be dried before use.

The Cystatin C Assay Kit provides an easy-to-use ELISA test. It is a quantitative sandwich enzyme immunoassay, which means that it is a quantitative, standardized method. After the samples have been sampled, the results are presented in a single color-coded format. The analysis is done with a reagent. A suitable standard is a good reference.

The Human Cystatin C Standard is 15 ng of native human Cystatin C in a buffered protein base. It can be reconstituted with 1 mL of 1x Assay Buffer. The Human Cystatin standard can be stored at -20 o C for one month without any problems. The Wash Buffer is 30 mL of diH2O. The reagent and the wash buffer are mixed together.

The Human Cystatin C Assay Kit uses a quantitative sandwich enzyme immunoassay. The samples are pipetted into wells that are coated with biotinylated detection antibody. After the samples have been separated, unbound proteins are removed. The HRP enzymatic reaction is carried out and the product of the test is a blue color. The yellow coloration is due to the amount of human Cystatin C captured in the plate.

The Human Cystatin C Standard is 15 ng of native Human Cystatin C in a buffered protein base. It can be stored at -20 o C for one month and should not be subjected to freeze-thaw cycles. Its corresponding test kit requires 30 mL of the 10x Assay Buffer. This buffer is used to remove the unbound antibody. The Human Cystatin C Assay Kit is available in multiple formats.
 

The Cystatin C Assay Kit utilizes a sandwich ELISA. This kit is designed to detect the amount of human cystatin C in a sample within an hour. The assay uses a specialized sandwich ELISA with a double antibody that is specific for Cystatin C. The result shows that the antibodies have been properly conjugated to the anti-CYS anti-antibody.

The Cystatin C Assay Kit from Diazyme Laboratories is a 4.5-hour solid-phase ELISA. This method uses a human NS0-expressed recombinant Cystatin C as a target. Its linear curves show the level of human Cystatin C in different samples. This method is very accurate and reproducible. The assay kit has been cleared by the FDA.