A High-Quality Adenosine Deaminase Assay Kit

Adenosine Deaminase (ADA) is a natural enzyme that is involved in the immune response. The function of Adenosine Diphosphate (ADP) is to activate the cells and perform membrane permeabilization and cytotoxic effect. Adenosine has been identified as one of the most important amino acids required for normal human brain activity. It is synthesized by the liver and is distributed in blood plasma through actions of the immune system. Adenosine Diphosphate is a carrier of the anti-viral genetic material. In Biomedical Expo, the ADA Activity Assay Kit will be used in combination with the previously mentioned BioVision Cytokine.

Adenosine diphosphate (ADP) is a carrier of T-lymphocyte growth factor. In the BioVision Cytokine, adenosine deaminase (ADO) is extracted from the dissected kidney and analyzed using commercially available spectrophotometric automated assay. The reagent was pre-treated with anti-phospho-dGlucose (PI) to prevent its reaction with the analyte.

The composition of the commercially available AIDA Activity Assay Kit includes two main components - the reagent and the laboratory equipment. The reagents contained in the Aida Activity Assay Kit include purified enzyme cocktail (Sigma-FCA). The laboratory equipment consists of a microplate scanner (mounted behind a glass microscope slide), glassware, standard curve results book, and rinse bottle. Before usage, the samples of the Aida Activity Assay Kit must be washed with de-chlorinated water. The use of reagent and the laboratory equipment is not restricted to laboratory tests only.

To examine the ability of the Aida Activity Assay Kit to measure total adenosine deaminase activity, several experimental sessions were conducted. The first session consisted of four days of manual tracing. One hundred and fifty microliters of Aida-GFP was dissolved in tap water and mixed with phosphate-buffered saline solution. After twenty minutes, the samples were tested on the HPLC mass-balance screen.

The second session included two additional days of manual tracing. Samples were injected onto the curve cassette for two hours as described above. The third session included three additional days of manual tracing in the same room, followed by four hours of subcutaneous injections of adenosine deaminase (ADCA). The blood sample was drawn after one hour for analysis.

For the saliva testing, five hundred microliters of standard solutions of deaminase and pH rose was mixed in a non-sterile punch. The pigs were allowed to drink a whole range of liquids, from plain water to diet soda, as desired. When the standard solutions reached their boiling point, a dropper with a pre-determined volume of saliva was inserted and the adenosine deaminase activity was measured.

The last group of six samples was analyzed using a commercially available Saliva-based Phosphorimidazoleamine Assay Kit (Saproflavil; Thermo Instruments). In this set of assays, a single drop of Phosphorimidazoleamine/salivary ada reagent was mixed with a volume of tap water and the dried powder was added to the samples. After thirty minutes, a second sample of the same sample was taken. In this case, the activity in the saliva was measured immediately. The kit had been specifically designed for measuring the activity of the acid in human saliva. As expected, there was a significant amount of activity that could be detected.

As expected, both analyzers produced similar results. The highly specific nature of the Salivary-Positron Emission Tomography (SPECT) analysis, together with the highly sensitive methodology (high intra-oral fluid detection) resulted in consistently high accuracy and significant measurements. The laboratory personnel used for the procedures were all experts in the field. There was no evidence of contamination, or of any kind of experiment error. The test subjects gave informed consent and the procedure followed the recommendations laid down by the American Association of Clinical Endocrinologists. Overall, the results obtained from the Adenosine Deaminase Assay Kit showed good reproducibility, which was confirmed by separate studies from independent clinical laboratories.